Detection of phomopsis canker and dieback of highbush blueberries and cranberries in Lithuania
Kacergius, A.(Institute of Botany, Vilnius (Lithuania). Lab. of Phytopathogenic Microorganisms)E-mail:[email protected] | Gabler, J.(Federal Centre for Breeding Research on Cultivated Plants, Aschersleben (Germany). Inst. for Resistance Research and Pathogen Diagnostics)E-mail:[email protected]. | Jovaisiene, Z.(State Plant Protection Service, Vilnius (Lithuania). Lab. of Phytosanitary Research)
Twig canker and blight caused by Phomopsis vaccinii Shear in Shear, N. Stevens and H. Bain (telemorph Diaporthe vaccinii Shear in Shear, N. Stevens and H. Bain) is a harmful disease of highbush blueberry and cranberry (Vaccinium spp.) and is under strong phytosanitary control in Lithuania as well in Europe and Mediterranean region. In 2002 the Phomopsis vaccinii on blueberry was identified for the first time in Lithuania. The diagnosis of this disease using classical phytopathological methods is often difficult because of overgrowing of target fungus by another species mostly necrotrophs or another canker agent Fusicoccum putrefaciens Shear. Only in one specimen the sporificated pycnidia with a- and a-conidia were observed on diseased plant. Exact Phomopsis vaccinii detection was succeeded using serological method, such as PTA-ELISA. The PTA-ELISA was performed using polyclonal IgG 59/3, specific for the genus Phomopsis. First results show positive reaction in 8 specimens. Development of the present serological diagnostic method could be very promising for the fast and exact identification of harmful disease agent Phomopsis vaccinii.
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