Characterization and Transcriptional Expression of the α-Expansin Gene Family in Rice
2005
Shin, J.H. (Pohang University of Science and Technology, Pohang, Republic of Korea) | Park, M.C. (The Catholic University of Korea, Bucheon, Republic of Korea) | Jeong, D.H. (Pohang University of Science and Technology, Pohang, Republic of Korea) | An, G.H. (Pohang University of Science and Technology, Pohang, Republic of Korea), E-mail: [email protected]
The rice genome contains at least 28 EXPA (α-expansin) genes. We have obtained near full-length cDNAs from the previously uncharacterized genes. Analysis of these newly identified clones together with the 12 identified earlier showed that the EXPA genes contain up to two introns and encode proteins of 240 to 291 amino acid residues. The EXPA proteins contain three conserved motifs: eight cysteine residues at the N-terminus, four tryptophan residues at the C-terminus, and a histidine-phenylalanine-aspartate motif in the central region. EXPA proteins could be divided into six groups based on their sequence similarity. Most were strongly induced in two-day-old seedlings and in the roots of one-week-old plants. However, only 14 genes were expressed in the aboveground organs, and their patterns were quite diverse.
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