Diversity in the spore print of the hybrid of Lentinula and Pleurotus on the basis of nuclear DNA content
2005
Kullman, B.(Estonian Agricultural Univ., Tartu (Estonia). Inst. of Agricultural and Environmental Sciences)E-mail:[email protected] | Greve, B.(University of Muenster (Germany). Inst. of Radiobiology) | Severin, E.(University of Muenster (Germany). Inst. of Radiobiology)
Four spore prints of the strains of Lentinula edodes L18, L. edodes L19, Pleurotus ostreatus x P. ostreatus P403xP404 and L. edodes x P. ostreatus L19-1xP404 were studied with flow cytometry. Diversity was found in the spore print of the interspecies hybrid fruit body L19-1xP404 and in the spore print of the fruit bodies of the parental strains P403xP404 and L19. One fruit body can produce two more or less equality represented subpopulations of spores with different nuclear DNA and protein contents. There exist subpopulations with similar DNA content among the spore prints produced by parental and hybrid fruit bodies. The dikaryon stage in the fungal life cycle allows for the genomes of different species to coexist and to event produce a fruit body. Diversity of the nuclear DNA content of a spore print reflects the fate of the hybrid genome during meiosis. After karyogamy, this fruit body distributes viable spores. It means that meiosis can even occur in the case of low density of homology between the chromosomes (CLP and aneuploidy) ensuring distribution of highly different genome sizes of strains.
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