Influence of physical factors and various complex media on growth and bacteriocin production of two-synergistic peptide with heat stable bacteriocin producer, Enterococcus faecium NKR-5-3, isolated from Thai fermented fish
2002
Pongtep Wilaipun(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agro-Industry. Department of Biotechnology) | Zendo, Takeshi(Kyushu University, Fukuoka (Japan). Faculty of Agriculture. Dept. of Bioscience and Biotechnology) | Mathana Sangjindavong(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Fisheries. Dept. of Fishery Products) | Sunee Nitisinprasert(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agro-Industry. Department of Biotechnology) | Vichien Leelawatcharamas(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agro-Industry. Department of Biotechnology) | Nakayama, Jiro(Kyushu University, Fukuoka 812-8581 (Japan). Faculty of Agriculture. Dept. of Bioscience and Biotechnology) | Sonomoto, Kenji(Kyushu University, Fukuoka (Japan). Faculty of Agriculture. Dept. of Bioscience and Biotechnology)
Enterococcus faecium NKR-5-3, isolated from Thai fermented fish (Pla-ra), produced an interested two-synergistic peptide bacteriocin with heat stable and broad spectrum activity. The influence of complex media and culture condition on growth as well as bacteriocin production of E. faecium NKR-5-3 has been studied. From 8 indicator strains used, Enterococcus faecalis ATCC 19433 was found to be the most sensitive strain. Bacteriocin to be diluted with distilled water containing 0.1 percent (v/v) Tween 80 increased 2-fold to 4-fold of its activity against indicator strains when compared with distilled water. In M-MRS broth, bacteriocin production reached maximum level at 30 deg C and decreased with the increasing of culture temperature. Among different 6 complex media for lactic acid bacteria cultured at 30 deg C, M 17 broth with the initial pH of 7.5 yielded the maximum growth and bacteriocin production of E. faecium NKR-5-3. Growth and bacteriocin production decreased when it was cultured in M 17 broth containing more than 1 percent NaCl and they were completely inhibited in M 17 broth containing more than 9 percent NaCl. Bacteriocin activity reached a detectable level at the early exponential phase and increased due to the cell growth to the maximum level at the end of exponential phase in 22 hr of incubation, which displayed a primary metabolite production.
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