[Study of sterilization schemes during the initiation of in vitro culture of red raspberry]
2006
Volosevich, N.N.,National Academy of Sciences. Scientific and Practical Center for Potato, Vegetable and Fruit Growing (Belarus). Institute for Fruit Growing
Obtaining sterile plant material plays an important role in initiation of red raspberry in vitro culture. 0,1% mercuric chloride solution, 0,1% silver nitrate solution and 6% hydrogen peroxide solution were tested as sterilizing agents. The following sterilization scheme was optimum: washing in running water during 1 hour, treatment by 70% ethanol during 5 sec., then by 0,1% solution of mercuric chloride during 3 minutes, and afterwards by three-time washing with sterile water (three times by 5 minutes), that provided maximal output of regenerants with low level of infection
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