Physiological mechanism of metabolism of carbohydrate, phenols, free amino acid and endogenous hormones in middle scales of Lilium davidii var. Unicolor bulbs stored at low temperature for dormancy release
2005
Sun Hongmei,Li Tianlai,Li Yunfei
中国人. 以兰州百合为试材，研究了2℃、6℃、10℃条件下保湿贮藏101 d内兰州百合中部鳞片中碳水化合物、酚类物质、游离氨基酸以及内源激素的变化对解除休眠的影响。结果表明，随着处理温度降低和处理时间延长，淀粉明显降解、ABA含量显著下降，可溶性糖、酚类物质、内源GA3、IAA含量急剧增加。低温处理初期的34 d是上述物质变化最活跃的时期。相关分析表明，淀粉酶和PAL活性升高是分别导致可溶性糖和酚类物质含量增加的因素。低温贮藏期间，游离氨基酸总量呈下降趋势，其中精氨酸含量最高、变化最大。根据逐步回归分析和通径分析的结果初步判定鳞片内碳水化合物、酚类物质和游离氨基酸等物质变化主要受内源GA3的调控。
显示更多 [+] 显示较少 [-]英语. Changes in carbohydrate, phenols, free amino acids and endogenous hormones in middle scales of Lilium davidii var. unicolor bulbs stored in moist sawdust for 101 days at 2℃, 6℃ and 10℃, respectively, were studied. Results indicated that starch content and ABA level decreased markedly along with decline of storage temperature and prolonging of duration, and total soluble sugar content，phenols content, GA3 and IAA concentration increased sharply. Notable changes of above substances occurred at earlier phase (34 d). Correlation analysis revealed that increase of amylase activity and PAL activity conduced to increase of total soluble sugar content and phenols content, respectively. As a general tendency, free amino acid content decreased, and arginine content was the highest and its changes were dramatic among all kinds of free amino acids. According to results of stepwise regression analysis and path analysis, it is concluded preliminarily that the metabolism in middle scales may be regulated mainly by endogenous GA3.
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