Research on compatibility effect between pretreatment extenders and cryoprotective extenders in frozen boar semen
2006
Hu Jianhong | Li Qingwang | Jiang Zhongliang
中国人. 采用6种预处理稀释液对猪精液进行预处理，然后用3种冷冻基础液冷冻保存，研究精液预处理稀释液和冷冻基础液对精子冷冻保存效果的影响，以及其间的配伍效应。结果表明，不同的预处理稀释液对解冻后精子活力的影响差异显著(P＜0.05)，而3种冷冻基础液对冻后精子活力的影响差异不显著（P＞0.05）。不同的预处理稀释液与冷冻基础液之间存在明显的配伍效应，并且用含甘油的稀释液对平衡不同时间的精液冷冻时，其配伍效应又有所不同。猪精液中去除精清可显著提高精子冻后活力和顶体完整率(P＜0.05)，有利于提高精液品质。
显示更多 [+] 显示较少 [-]英语. The purpose of this study was to research the effect of pretreatment extenders and cryoprotective extenders on frozen boar semen and their co-effect. Six home-temp extenders were used in pretreatment and three cryoprotective extenders were used in cryo-preservation in this experiment. The results were as follows: there was significant difference in the frozen-thawed sperm motility among six pretreatment extenders (P＜0.05), however, there was no significant difference in the frozen-thawed sperm motility among three cryoprotective extenders (P＞0.05). Significant difference existed in compatibility effect between pretreatment and cryoprotective extenders(P＜0.01). Different balancing time led to different compatibility effects. The motility and acrosomal integrity of frozen-thawed sperm could be significantly improved by removing the seminal plasma.
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