Cloning and sequencing of Sinkiang isolate of apple scar skin viroid (ASSVd)
2006
Zhao Ying | Niu Jianxin
中国人. 利用自行设计的方法分离提取总RNA，通过一对特异引物，扩增出苹果锈果类病毒的全长片段，该片段通过回收、克隆和测序，结果发现该片段全长330bp，与已发表的NC-001340同源性为99．7％，仅在第126有一个碱基差异。由此证明该片段是苹果锈果类病毒的全长序列，进一步证明该反应体系能很好地用于苹果锈果类病毒的RT―PCR检测，为快速鉴定类病毒奠定了良好基础。
显示更多 [+] 显示较少 [-]英语. The method designed by ourselves was used for the isolation of total RNA. The fragment of ASSVd was amplified by a pair of specific primer. Gel extraction, cloning and sequencing of the fragment revealed it was 330 bp long with a 99.7% level of homology and only a difference in 131 bp site compared with NC 001340 published before. Thus it proved that the fragment can represent the full length of the sequences of ASSVd. It also further proved that the reaction system can be well used to detect ASSVd by RT-PCR (reverse transcription-polymerase chain reaction) which established good foundation for rapid detection of viroid.
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