Protein patterns associated with Pisum sativum somatic embryogenesis
2007
Griga, M.,AGRITEC, Sumperk (Czech Republic). Oddeleni Biotechnologie | Horacek, J.,AGRITEC, Sumperk (Czech Republic). Oddeleni Biotechnologie | Klenoticova, H.,AGRITEC, Sumperk (Czech Republic). Oddeleni Biotechnologie
Total protein patterns were studied in the course of development of pea somatic embryos (SE) using simple protocol of direct regeneration from shoot apical meristems on auxin supplemented medium. Protein content and total protein spectra (SDS-PAGE) of SE in particular developmental stages were analysed in Pisum sativum, P. arvense, P. elatius and P. jomardi. Expression of seed storage proteins in SE was compared with their accumulation in zygotic embryos. Pea vegetative tissues, namely leaf and root, were used as a negative control not expressing typical seed storage proteins. The biosynthesis and accumulation of seed storage proteins was observed during SE development (since globular stage), despite of the fact that no special maturation treatment was applied. Major storage proteins typical for pea seed (globulins legumin, vicilin, convicilin and their subunits) were detected in SE. In general, the biosynthesis of storage proteins in SE was lower than in mature dry seeds. However, in some cases the cotyledonary SE exhibited comparatively high expression of vicilin, convicilin and pea seed lectin, which was even higher than those in immature but morphologically fully developed zygotic embryos. Desiccation treatments did not affect the protein content of SE. The transfer of desiccated SE on hormone-free germination medium led to progressive storage protein degradation.
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