Relationship between abnormal spermatozoa and seminal plasma free amino acids in boars
2007
Cerovsky, J.,Vyzkumny Ustav Zivocisne Vyroby, Kostelec nad Orlici (Czech Republic) | Frydrychova, S.,Vyzkumny Ustav Zivocisne Vyroby, Kostelec nad Orlici (Czech Republic) | Lustykova, A.,Vyzkumny Ustav Zivocisne Vyroby, Kostelec nad Orlici (Czech Republic) | Rozkot, M.,Vyzkumny Ustav Zivocisne Vyroby, Kostelec nad Orlici (Czech Republic)
Semen of 37 experimental adult boars kept in the same conditions was used in order to evaluate the relationship between seminal plasma free amino acids (FAAs) and total contents of morphologically abnormal spermatozoa (MAS). Immediately after collection, semen gel free volume, spermatozoa concentration and total MAS were determined microscopically. The boars were divided into two groups according to the significantly different total MAS content as follows: group A: n=24, 9.46+/-4.95% of MAS; group B: n=13, 46.00+/-14.54% of MAS. Deproteinized seminal plasma samples were analysed for concentrations of 13 FAAs: Tau, Asp, Thr, Ser, Glu, Gly, Ala, Val, Met, Ile, Leu, Tyr and Lys by liquid chromatography (AAA 339 M analyser). Glutamic acid was a predominant FAA in seminal plasma in both groups of boars. Its concentration was significantly higher in A boars (28.49 vs. 17.60 microM/100 mL), but its proportion in the total content of FAAs was nearly equal in both groups of boars (A=38.1%, B=38.9%). The differences in the particular FAAs recorded between the two groups were statistically significant for 11 out of the 13 FAAs in favour of the group A as well as for the total concentration of FAAs per boar (74.70 vs. 45.23 microM/100 mL). A highly significant correlation was determined between the total MAS and total FAA concentration in all experimental boars (r=-0.60).
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