Isolation of conditionally pathogenic mycobacteria from the environment of one pig farm and the effectiveness of preventive measures between 1997 and 2003
2007
Pavlik, I.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Matlova, L.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Gilar, M.,Krajska Veterinarni Sprava, Pelhrimov (Czech Republic) | Bartl, J.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Parmova, I.,Statni Veterinarni Ustav, Prague (Czech Republic) | Lysak, F.,Field Veterinarian, Pelhrimov (Czech Republic) | Alexa, M.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Dvorska-Bartosova, L.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Svec, V.,Krajska Veterinarni Sprava, Pelhrimov (Czech Republic) | Vrbas, V.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Horvathova, A.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)
Between 1997 and 2003, positive and dubious responses to avian tuberculin were detected in 4 and 72 pigs, respectively, in a breeding herd of 90 sows and 2 boars. Pigs were examined using the agglutination test for the presence of serum antibodies against corpuscular antigens prepared from various Mycobacterium avium complex (MAC) members: M. a. avium (MAA) of serotype 2, M. a. hominissuis (MAH) of serotype 8 and M. intracellulare (MI) of serotype 19. Positive skin responses were found in animals with antibodies against MAH (23.7%), MAA (4.0%) and MI (11.8%) antigens. By serological examination of 17 sows with repeated dubious responses for tuberculin skin testing with avian tuberculin, no antibodies against MAA were detected; MAH antibodies and MI antibodies were found in eight and two animals, respectively. No tuberculous/tuberculoid lesions were detected by postmortem examination of lymph nodes (ln) and organ samples from all 76 animals with responses to avian tuberculin. Culture examination of ln and organs from 13 animals revealed conditionally pathogenic mycobacteria (CPM) in one boar. These isolates were identified as MAH and CPM by the PCR method and biochemically. Investigation of the external environment (205 samples) revealed 16.3% CPM isolates as follows: 13 MAH, 8 M. fortuitum, one M. nonchromogenicum, one M. abscessus, one M. scrofulaceum and 9 unidentified isolates which were non-MAC according to the PCR examination. Animal hygiene measures adopted since 2002 resulted in a decrease of environmental contamination with CPM and a reduction in the number of animals with positive responses to avian tuberculin.
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