Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
2007
Krizkova, S.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie | Zitka, O.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie | Adam, V.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie | Beklova, M.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Veterinarni Ekologie a Ochrany Zivotniho Prostredi | Horna, A.,Univerzita Tomase Bati, Zlin (Czech Republic). Ustav Potravinarskeho Inzenyrstvi | Svobodova, Z.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Parazitologie | Sures, B.,Universitaet Duisburg-Essen (Germany). Angewande Zoologie/Hydrobiologie | Trnkova, L.,Masarykova Univ., Brno (Czech Republic).Ustav Chemie | Zeman, L.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Vyzivy Zvirat a Picninarstvi | Kizek, R.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie
In the present paper, we report on the use of adsorptive transfer stripping technique in connection with chronopotentiometric stripping analysis for metallothionein (MT) determination and of differential pulse anodic stripping voltammetry for lead detection in tissues (muscles, gonads, liver and spleen) of wild perch (n=6) from the Svratka River in Brno, Czech Republic. The highest MT concentrations were detected in spleen and liver (100-350 ng MT per gram of fresh weight). We assume that the content of MT determined in perch tissues is probably related with the age of the fish and therefore with their exposition to heavy metals naturally occurring in the Svratka River. MT concentrations were very well correlated with Pb concentrations.
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