Large scale micropropagation of sugar beet by using priodical bioreactor
2004
Roozbeh, Faranak | Davoodi, Darioosh | Mesbah, Mahmood
Selected genotypes of sugar beet are often maintained by conventional propagation, but results are not always satisfactory and much faster rates of multiplication can be now obtained through micropropagation. On the other hand, plant micropropagation by using bioreactors can reducees the cost of production and makes it economical. In this research, the possibility of using bioreactor and optimization of the tissue culture procedure in sugar beet micropropagation has been studied. At first, a simple periodical bioreactor was made by using a timer, an air pump, microfilters (0.2 µ), and autoclaveable transfer pipes. In vitro stock plantlets from seeds and liquid MS basal medium supplemented with 1.5 mgL-1 BAP, 0.2 mgL-1 NAA, 0.5 mgL-1 GA3, and 3% sucrose were used to inoculation and multiplication in the bioreactors. Periodical nutrition of the explants established for 10 minutes in each 6 hours. The same basal medium with 3 mgL-1 IBA was used for root induction. Comparison was made for multiplication rate in bioreactor and routin in vitro culture and propagation in four replications. Results showed that multiplication rate, number of shoots, and biomass production in the bioreactor system are significantly different from those of routine in vitro culture in 100 ml jars. But rooting percentage in bioreactor was very low and needs more study for optimization.
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