Molecular marker assisted selection in breeding for resistance to bacterial wilt in carnation (Dianthus caryophyllus L.)
2006
Yagi, M.(National Inst. of Floricultural Science, Tsukuba, Ibaraki (Japan)) | ; Onozaki, T. | ; Tanikawa, N. | ; Shibata, M.
The availability of marker-assisted selection (MAS) using DNA markers close to QTLS of resistance to bacterial wilt in carnations (Dianthus caryophyllus L.) was examined. The STS-WG44 marker tightly linked to the major resistant gene was detected in all backcross lines with resistance selected by the root-soaking method. Quantities of markers OQ12 and STS-WB66 close to QTLS with slight effects in resistant lines were lower in succeeding generations of backcrossing. These findings suggested that STS-WG44 is available for selecting resistant lines. In practical breeding populations, the difference of mean disease incidence between two groups categorized as having or lacking STS-WG44 was 62.6% and STS-WG44 was possessed in most lines showing resistance less than 20% in disease incidence. These findings suggest that STS-WG44 as a selective marker facilitated narrowing of populations to those that are highly resistant for practical breeding. Marker-assisted selection would be available for breeding improved resistance to bacterial wilt in carnations.
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