Root formation via calli derived from mesophyll cells of peanut (Arachis hypogaea)
2006
Usui, H.(Mie Univ., Tsu (Japan). Faculty of Bioresources) | ; Fukushima, K. | ; Ueda, Y.
2-3 X 10E6 mesophyll cells could be isolated enzymatically from mature leaves of 10 peanut varieties (Arachis hypogaea cv. Chiba No.43, Chiba handachi, Jawa No.13, Hotaku churyu, Nakateyutaka, Kanto No.56, Chiba shoryu, Enomame, T. M. V.-3, Krapovickas-I). The cells devided, grew actively on a solid, modified NT medim (1) (3.0 mg/l NAA, 1.0 mg/l BA, no osmoticum) and formed calli after 3 weeks of culture. The calli were transplanted on MS solid media (2) (0.5 or 2.0 mg/l 2,4-D and 0.01, 0,15 or 2.25 mg/l kinetin) . Some roots were regenerated from the calli of Arachis hypogaea cv. Chiba handachi on medium (2) containing 2.00 mg/l 2,4-D and 0.01 or 0.15 mg/l kinetin within 4 weeks of culture. Differences in growth activities of mesophyll cells and root formation from their calli among 10 peanut varieties were discussed.
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