Effect of addition of betaine in the medium of in-vitro production of bovine blastocysts
2006
Tohyama, M.(National Livestock Breeding Center, Tamana, Kumamoto (Japan). Kumamoto Station) | ; Hirata, S. | ; Kai, K. | ; Tsunoda, M. | ; Hashimoto, M. | ; Goto, H. | ; Iwashita, T. | ; Hirai, T.
We investigated the effect of addition of betaine in the media in in-vitro production of bovine blastocysts. Addition of betaine in the media improved the cell number and quality of blastocysts. The effective concentration of betaine was 3 mM. However, it was necessary to add 3 mM betaine in all culture media through in-vitro maturation, fertilization and culture. The pregnancy rate after embryo transfer was 31.8% in the bovine blastocysts that were produced in the media with added 3mM betaine. The average body weight of calves was 35.4+-4.6kg without abortion and stillbirth in these pregnancy. Also no large calves were born from this method. Therefore, addition of betaine in the media in in-vitro production of bovine blastocysts has no detrimental effect on newborn calves. The present study suggested that addition of betaine in the media could improve the rate of incidence and the quality of bovine blastocysts produced in in-vitro.
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