Antihypertensive effect of angiotensin I-converting enzyme inhibitory peptides from a sesame [Sesamum indicum] protein hydrolysate in spontaneously hypertensive rats
2006
Nakano, D.(Osaka Univ. of Pharmaceutical Sciences, Takatsuki (Japan)) | Ogura, K. | Miyakoshi, M. | Ishii, F. | Kawanishi, H. | Kurumazuka, D. | Kwak, C.J. | Ikemura, K. | Takaoka, M. | Moriguchi, S. | Iino, T. | Kusumoto, A. | Asami, S. | Shibata, H. | Kiso, Y. | Matsumura, Y.
Sesame peptide powder (SPP) exhibited angiotensin I-converting enzyme (ACE) inhibitory activity, and significantly and temporarily decreased the systolic blood pressure (SBP) in spontaneously hypertensive rats (SHRs) by a single administration (1 and 10mg/kg). Six peptide ACE inhibitors were isolated and identified from SPP. The representative peptides, Leu-Val-Tyr, Leu-Gln-Pro and Leu-Lys-Tyr, could competitively inhibit ACE activity at respective Ki values of 0.92 microM, 0.50 microM, and 0.48 microM. A reconstituted sesame peptide mixture of Leu-Ser-Ala, Leu-Gln-Pro, Leu-Lys-Tyr, Ile-Val-Tyr, Val-Ile-Tyr, Leu-Val-Tyr, and Met-Leu-Pro-Ala-Tyr according to their content ratio in SPP showed a strong antihypertensive effect on SHR at doses of 3.63 and 36.3 microg/kg, which accounted for more than 70% of the corresponding dosage for the SPP-induced hypotensive effect. Repeated oral administration of SPP also lowered both SBP and the aortic ACE activity in SHR. These results demonstrate that SPP would be a beneficial ingredient for preventing and providing therapy against hypertension and its related diseases.
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