Diversity Analysis of Japonica Rice using MITE-transposon Display
2006
Hong, S.M. (Chungnam National University, Daejeon, Republic of Korea) | Kwon, S.J. (Rural Development Administration, Suwon, Republic of Korea) | Oh, C.S. (Chungnam National University, Daejeon, Republic of Korea) | Wessler, Susan R. (University of Georgia, Athens, GA, USA) | Ahn, S.N. (Chungnam National University, Daejeon, Republic of Korea), E-mail: [email protected]
Miniature inverted transposable elements (MITEs) are abundant genomic components in plant including rice. MITE-transposon display (MITE-TD) is an Amplified Fragment Length Polymorphism (AFLP)-related technique based on MITE sequence. In this study, we used the MITE-AFLP for the analysis of diversity and relationship of the 114 japonica accessions. Of the several MITEs, the mPing family was applied to detect polymorphisms based on PCR amplification. The BfaI adaptor primer and the specific primer derived from mPing terminal inverted repeat (TIR) region were used to PCR amplification of 114 accessions.
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