Functional Implication of the tRNA Genes Encoded in the Chlorella Virus PBCV-1 Genome
2005
Lee, D.Y. (Pukyong National University, Busan, Republic of Korea) | Graves, Michael V. (University of Massachusetts-Lowell, Lowell, MA, USA) | Van Etten, James L. (University of Nebraska, Lincoln, NE, USA) | Choi, T.J. (Pukyong National University, Busan, Republic of Korea), E-mail: choitj@pknu.ac.kr
The prototype Chlorella virus PBCV-1 encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative virus attachment protein, in E. coli strain BL21(DE3) SI were unsuccessful, and multiple protein bands were detected on Western blots. However, the full-length A189/192R recombinant protein or fragments derived from it were detected when they were expressed in E. coli BL21 CodonPlus (DE3) RIL, which contains extra tRNAs.
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