Assessment of the impacts of probiotics in shrimp (Penaeus monodon Fabricius) culture
2005
Calpe, A.T.
The impacts of using probiotics in shrimp culture was studied. Shrimps were cultured in tanks using a completely randomized block design with two treatments. Four tanks were treated with probiotics and the other four tanks serve as control. Water quality parameters were monitored in the tanks and in the discharge canal from July to Dec 2003. All the water quality parameters fell within the optimum water quality standards for shrimp culture and only pH was significantly different between treatments. Counts of luminous bacteria, total Vibrio, yellow colony bacteria, and green colony bacteria were not significantly different between treatments. Shrimps treated with probiotics showed a significantly improved growth rate and survival rate. The DGGE [ denaturing gradient gel electrophoresis] profile of the commercial probiotics (BZT Aqua) using the said 341fGC/534r primer pair and a 20-60 percent denaturing condition was obtained. Bands that were su ccessfully sequenced revealed that the closest phylogenetic relatives of the species present in the commercial probiotics included Enterococcus sp., Bacillus sp., Bacillus licheniformis, Streptomyces sp., and an uncultured bacterium clone. Probiotics also contributed to the change in species composition and improved shrimp production. The use of probiotics in shrimp farming was found to be profitable with a return on investment of 89%.
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