Generation and evaluation of doubled haploid lines (DHLs) for improved grain quality and resistance to pests
2005
Anon.,Philippine Rice Research Inst., Maligaya, Science City of Munoz, Nueva Ecija (Philippines)
Anther culture technique is being used as an adjunct to conventional breeding in an attempt to hasten rice varietal development. The anther culture technology can generate homozygous lines in one generation. This can shorten the breeding cycle by 3 to 4 years. Selected F2S in the breeding program for special rice at UPLB [University of the Philippines Los Banos, College, Laguna, Philippines] and PhilRice were used as anther sources. The materials were selected fo their exceptional attributes, i.e. aroma, resistance to tungro and BLB, and high yield. The DHLs generated from anther culture were evaluated in the greenhouse and field. During 2003 WS, 66 DHLs were generated from 54, 360 anthers plated. Seed materials were increased in the greenhouse in 2004 DS and evaluated under the ON in 2004 WS. These lines were uniform in terms of days to heading, plant height, leaf, and stem color. All the lines were selected and advanced to PYT in 2005 DS and the performance of these lines will be compared with the check varieties PSB Rc18 and IR72. From 148,680 anthers plated during 2004 DS, 152 DHLs were generated. Seeds of these lines were increased in the greenhouse in 2004 WS. Following the panicle to a row method, these DHLs are now planted in the field under the 2005 DS ON for evaluation. To date, 105 DHLs have been generated from the 125,820 anthers plated during 2004 WS. At least 45% of the calli induced from these anthers are still in the regeneration phase. The DHLs are now planted in the greenhouse for initial seed increase.
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