Getting close to the salt tolerant gene ++
2004
Tumimbang, E.B.,International Rice Research Inst., DAPO Box 7777, Metro Manila (Philippines). Plant Breeding, Genetics and Biochemistry Div.) | Niones, J.M. | Adorada, D.L. | Gregorio, J.B. | Elahi, F. | Seraj, Z.
The analysis of quantitative trait loci (QTLs) associated with salinity tolerance was carried out using a population of near-isogenic lines (NILs) BC3F4 derived from Pokkali as donor and IR29 as recurrent parent (IR29/Pokkali//3*IR29). The NILs were developed to fine map the major QTL for salinity tolerance in chromosome 1 of rice. Twenty-six plants composed of three families were selected to generate 10,000 BC3F4 plants to fine map the salinity tolerance gene in chromosome 1. Seven microsatellites (RM8115, RM140, RM493, RM8094, RM3412, AP3206 and AP3722) and three EST markers (CP6224, CP010136 and CP03970) were found within the region of interest and were used to saturate the region where the,major QTL for salt tolerance was mapped. Linkage and graphical genotype analyses were done using the computer program, MapmaRker and Qgene, respectively. Each markers and trait (tolerance score) were subjected to QTL detection based on single marker analysis (one-way ANOVA) and correlation test. Two new markers, CP6224 and RM8094 flanking the previously mapped major QTL (Bonilla et al., 2002) at 5.75 cM interval distance were found significantly associated with the salinity tolerance trait. Genotyping of other families of the near-isogenic line population is on-going to further reduced the distances of the flanking markers which are significantly associated with salt toleranT gene. Markers highly associated with the trait will be used for marker-assisted breeding in developing salinity tolerant varieties.
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