In vitro propagation of selected cultivars and hybrids of Lilium sp.
2005
Vinterhalter, B.(Institut za bioloska istrazivanja Sinisa Stankovic, Beograd (Serbia)) | Vinterhalter, D.(Institut za bioloska istrazivanja Sinisa Stankovic, Beograd (Serbia))
Five commercial cultivars of Lilium, three of the group Orientals (Belcanto, Kasandra and Sol) and two Asiatic hybrids (with yellow and orange coloured flowers) were introduced in in vitro culture conditions by culturing explants from bulb leaves on the MS medium with 3% sucrose, 0.2 mg/l BA and 0.05 mg/l NAA. The efficiency of the bulblet production (proliferation) for five cultivars was appoximately equal to 70%. The bublet multiplication was continued on the same medium with sucrose increased to 5%. Prior to planting, bulblets were stored for two months in a cold room (+5 deg C) in darkness in media with 9% sucrose to breake the dormancy. The bulblet number, weight, dimensions and root production in darkness were measured. The largest bulblets (1343.35 mg) were formed by cv. Sol. Bulblets of Asiatic cultivars were much smaller than of cultivars of the group Orientals. After planting, the adaptation success was approximately 70% on the average. Plants flowered in spring some 18 months after planting.
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