Changes in the content of phenolic compounds in sunflower leaves after inoculation with the causal agent of downy mildew
2006
Radovanovic, N.(Naucni institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)) | Pucarevic, M.(Naucni institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)) | Jocic, S.(Naucni institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)) | Saftic-Pankovic, D.(Naucni institut za ratarstvo i povrtarstvo, Novi Sad (Serbia))
Two nearly isogenic lines of sunflower, resistant and susceptible to downy mildew, due to the presence/absence of the P16 gene, were used in this study. Inoculation with the suspension of Plasmopara halstedii zoospores, was done on the plants in the phase of the first pair of leaves. In the samples taken from 2nd to 96th h after inoculation, content of phenolic compounds in methanolic extracts from frozen leaves were analysed (UV-VIS and HPLC analysis). A constitutive level of phenolic compounds, as well as, their accumulation after the inoculation were higher in the susceptible than in the resistant isogenic line. Chlorogenic, ferulic acid and esculin had the highest contribution to the phenolic compound accumulation 24 h after the inoculation in the susceptible line, while only the chlorogenic acid and eculin accumulated in the resistant line. Also, there was a trend of scopoletin accumulation in the resistant line.
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