The cellular fatty acid composition of Bradyrhizobium japonicum and Sinorhizobium meliloti strains
2006
Gojgic-Cvijovic, G.(Institute of Chemistry, Tehnology and Metallurgy, Belgrade (Serbia). Department of Chemistry) | Radin, D.(Faculty of Agriculture, Belgrade - Zemun (Serbia)) | Milicic, B.(Institute of Soil Science, Belgrade (Serbia)) | Djokovic, D.(Faculty of Chemistry, Belgrade (Serbia))
The aim of this work is to analyse fatty acids profiles of Sinorhizobium meliloti and Bradyrhizobium japonicum strains and establish an eventual connection of this composition and nitrogen fixing effectiveness. The strains were isolated from soils of Serbia and obtained from collection of Institute of Soil Science, Belgrade (Serbia). Methyl esters of total fatty acids were prepared from lyophilised biomass using toluene-methanol-sulphuric acid mixture and analysed using GC-MS method. In Sinorhizobium meliloti strains the main components are 16:0, sigma 18:1, cy19:0 and in lesser proportion are cy 17:0, 17:0, 18:0, 16:1, 3-OH 14:0, 15:0 and 14:0 fatty acids. The ineffective strain (Nod+ Fix-) contains remarkable less cy17:0 acid. Dominant components in Bradyrhizobium japonicum strains are sigma18:1 and 16:0 fatty acids. Also cy19:0, 18:0 and 16:1 components were detected and certain shifts in the amount of these minor components observed. However, on the basis of our results there is no correlation between the relative content of particular fatty acids and the fixing effectiveness of tested strain.
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