The influence of cadmium on the in vitro growth of poplar shoots from the Leuce section
2006
Katanic, M.(Poljoprivredni fakultet, Novi Sad (Serbia). IRC Institut za nizijsko sumarstvo i zivotnu sredinu) | Tomovic, Z.(JP Vojvodinasume, Novi Sad (Serbia)) | Pilipovic, A.(Poljoprivredni fakultet, Novi Sad (Serbia). IRC Institut za nizijsko sumarstvo i zivotnu sredinu) | Orlovic, S.(Poljoprivredni fakultet, Novi Sad (Serbia). IRC Institut za nizijsko sumarstvo i zivotnu sredinu) | Krstic, B.(Prirodno-matematicki fakultet, Novi Sad (Serbia). Departman za biologiju i ekologiju)
The influence of cadmium presence on growth of shoots of four poplar clones from section Leuce cultivated in tissue culture is presented in this paper. Shoots of clones L-12, L-80, L-111/81 and LBM were multiplicated by micropropagation on ACM (Aspen Culture Medium) with 0.5 mg/l 6-benzylaminopurine (BAP) and 0.02 mg/l alfa-naphthaleneacetic acid (NAA). Terminal shoots, about 1.5 cm long, were cultivated 30 days on the ACM medium with addition of 0.1 mM and 0.5 mM of Cd. Parameters examined in shoot cultures included: length of the main shoot, shoot multiplication and fresh mass. It was observed that the addition of 0.5 mM was toxic for shoots of all investigated clones. The symptoms included were decrease of growth vigor and leaf chlorosis. Length, fresh mass and shoot multiplication were decreased compared to control. Low concentration of cadmium (0.1 mM) had stimulatory effects on fresh mass (in total) of investigated clones, especially for clone L-111/81. These results indicate possible use of investigated clones in phytoremediation of soils that contain lower concentration of cadmium, especially clone L-111/81 which was assumed as the most suitable for this purpose.
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