Effect of ACTH stimulation on cortisol and progesterone concentrations in intact and ovariohysterectomized domestic cats
2005
Kaywalee Chatdarong(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Obstetrics Gynaecology and Reproduction) | Suppawiwat Ponglowhapan(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Obstetrics Gynaecology and Reproduction) | Karlsson, Asa(Department of Biomedical Sciences and Veterinary Public Health, PO Box 7038, Uppsala (Sweden)) | Forsberg, Catharina Linde(Swedish University of Agricultural Science, Uppsala (Sweden). Faculty of Veterinary Medicine and Animal Science. Dept. of Clinical Science)
This study aimed to evaluate the adrenocortical capacity for cortisol and progesterone production in female cats, both while intact and after ovariohysterectomy. Five privately owned female cats aged 1-3 years were used in two trials while intact at an inactive stage of the cycle, and again in two trials at two-weeks after ovariohysterectomy. The four trials were: Intact saline-injection control trial, Intact ACTH injection (0.125), Ovariohysterectomized saline injection control trial, Ovariohysterectomized ACTH injection. Blood samples were obtained by indwelling cephalic vein catheter at -30, 0 (immediately before injections), and 60, 90, 120 and 180 min after injection. The mean basal pre-treatment concentrations of cortisol in the intact and ovariohysterectomized cats were 33+-19 and 32+-19 nmol/L, respectively. The corresponding values for progesterone were 1.1+-0.6 and 0.7+-0.6 nmol/L, respectively. Saline did not alter the serum cortisol or progesterone concentrations. In contrast, both cortisol and progesterone were elevated after ACTH, with peak values at 90 min and returned to basal levels at approximately 180 min. A positive correlation was found between cortisol and progesterone (r=0.8, p LT 0.05). In some instances the procedure used to restrain the cats during blood collection induced increases in cortisol and progesterone of the same magnitude as when the ACTH was administered. Such effects of restraint could alter the results of assisted reproduction efforts.
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