[Viability of potato plants in vitro. Analysis of problem and method of evaluation]
2007
Reutskij, V.G. | Rodionov, P.A. | Zubej, E.S. | Ashikhmina, N.S., National Academy of Sciences, Minsk (Belarus). The V.F. Kuprevich Institute of Experimental Botany
Investigation results showed that potato (Solanum tuberosum L.) explants in vitro should be classified as the special cultural phenotype characterized by the extremely low adaptive potential in the conditions of open ground. The speed of CO2 assimilation by potato leaves was lower than the nominal one, water transport in xylem was absent, stomatal regulation was inactivated, there were a lot of differences between potato morphological structure in vitro and the analogous plants cultivated in field conditions. Express method of plant viability was developed for the objective evaluation of the level of unsuitability of cultural phenotype to the requirements of ecological environment of the open ground. Developed in the Republic of Belarus original method of evaluation of potato plants viability in vitro conditions was based on the measurement of the level of aquaporines of a plasmatic membrane of leaf mesophyll cells. Express method was based on the analyses of curves of hydration and dehydration of the assimilative tissue. It was showed that the curve of starting segment of dehydration curve parallel to X axis was connected by the proportional dependence with speed level of symplastic water flow from apoplast into cell that made it possible to evaluate plant adaptive potential. The proposed method made it possible to define not only the level of plant viability but also to determine the beginning of plant stress condition. The method helped to evaluate the efficiency of impacts directed towards the increasing of explants quality
显示更多 [+] 显示较少 [-]