Induction of alternative oxidase chain under salt stress conditions
2008
Ferreira, A.L.,Universidade do Algarve, Gambelas (Portugal). Faculdade de Engenharia de Recursos Naturais | Arrabaca, J.D.,Universidade de Lisboa (Portugal). Faculdade de Ciencias | Vaz-Pinto, V.,Universidade de Lisboa (Portugal). Faculdade de Ciencias | Lima-Costa, M.E.,Universidade do Algarve, Gambelas (Portugal). Faculdade de Engenharia de Recursos Naturais
This paper describes the effect of NaCl on the respiration of Citrus cell suspensions namely on the induction of the alternative oxidase. The exposure of two Citrus (cvs. Carvalhal tangor and Valencia late) cell suspensions to 200 or 400 mM NaCl led to a reduction of cell respiration rates. Under these conditions, the respiration rate decreased less in the presence of KCN indicating a stimulation of the capacity of the alternative oxidase (AOX). In addition, immunoblots showed an increase in the amount of AOX protein. Antibodies raised against the Sauromatum guttatum enzyme recognized the reduced form of the enzyme near the 35 kDa band. The protein accumulation was correlated with the significantly higher AOX capacity observed for cv. Carvalhal tangor.
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