Evaluation on a number of soybean drought tolerance somaclones generated from in vitro selection
2004
Widoretno, W.((Universitas Brawijaya, Malang (Indonesia). Fakultas Pertanian)) | Sudarsono
The objectives of this experiments were to evaluate the response against drought stress of soybean somaclonal variants derived from in vitro selection of somatic embryos on medium containing polyethylene glycol (PEG), to identify drought tolerance somaclones, and to evaluate proline and total sugar content of the plants grown under water stress and non-stress conditions. For each SCJ soybean genotype, 12 SC2 progenies derived from its respective SCJ plants were grown in the plastic house under non-stress condition until flowering period. At the flowering stage, six plants were kept under non-stress conditions and the others were subjected to stress by watering the plants every four days. The stress treatment was conducted up to harvesting. Leaf proline and total sugar content were determined after 14 days of initial stress. During harvest, various vegetative and generative characters were measured and used to calculate drought sensitivity index and to determine the response of evaluated genotypes against drought. Results of the experiments indicated under the described stress condition, original soybean genotype B3731 was identified as medium tolerance while MLG2999 and MSC8606 were drought sensitive. On the other hand, a number of SC2 somaclones developed from somatic embryos regenerated on selective medium containg 20 percent PEG were identified as drought tolerance based on their drought sensitivity index. Drought tolerance somaclones were identified from B3731 (medium tolerance genotypes), MLG2999 and MSC8606 (drought sensitive genotypes) at the frequency of 55 percent, 11 percent, and 33 percent, respectively. Drought tolerance somaclones accumulated much higher proline than the original line or the drought sensitive somaclones. Total sugar accumulation showed no specific pattern associated with the response of the original genotypes or somaclones against drought stress.
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