Genetic Diversity in Iranian Onion Landraces Using RAPD Markers
2005
Musavi Zadeh, Ali | Turchi, Mahmud
In order to evaluate genetic diversity within and among Iranian onion landraces, twenty onion landraces along with two hybrids were Screened using random amplified polymorphic DNA (RAPD) markers. Twenty- six out of 200 tested RAPD primers produced 170 polymorphic bands. Within the 26 used primers, the total number of polymorphic bands within landraces and hybrids varied from 66 to 131 bands. Genetic distances among entries were ranged from 0.037 to 0.31 using Nei's genetic distance. The averages of total and within genetic diversity of landraces and hybrids were 0.341 and 0.234, respectively and the degree of genetic differentiation was estimated 0.31. Using Nei's gene index, gene diversity within landraces and hybrids was ranged from zero to 0.5. Molecular variance analysis based on squared Euclidean distances showed significant variance within and among onion landraces, but the variance between the entries (%25.7) was less than the variance within them (%74.3). Cluster analysis, grouped the entries into four clusters. Grouping based on molecular data showed no correspondence with the geographical pattern. Using principal coordinate analysis, the first three principal components accounted for %52.29 of the total data variation which showed suitable genomic distribution for used markers. This research showed that some of the Iranian onion landraces have potential source for different genes and this can be used in breeding programes.
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