Effect Of Hormons And Explant In Callus Induction And Plant Regeneration In Tissue Culture Of Anthurium Andreanum Var. Tropical

Ebrahimzadeh, M., Ph.D Student of Science & Research Unit,Tehran,Iran,; Shaker, H., cientific Member,Biology Department,Shahid Beheshti University,Tehran, Iran; Bernard, F., cientific Member,Biology Department,Shahid Beheshti University,Tehran, Iran; Khavarinezhad, R., Scientific Member,Biology Department Tarbiat Moalem University,Tehran, Iran

Effect Of Hormons And Explant In Callus Induction And Plant Regeneration In Tissue Culture Of Anthurium Andreanum Var. Tropical

2007

Ebrahimzadeh, M., Ph.D Student of Science & Research Unit,Tehran,Iran, | Shaker, H., cientific Member,Biology Department,Shahid Beheshti University,Tehran, Iran | Bernard, F., cientific Member,Biology Department,Shahid Beheshti University,Tehran, Iran | Khavarinezhad, R., Scientific Member,Biology Department Tarbiat Moalem University,Tehran, Iran

Anthurium andreanum var. Tropical from araceae family is one of the most beautiful flowers of the world. This plant was increased by seeds and planting of adventitious bud in the past but today these methods are not useful any more. In this research in vitro culture of leaf explant has been studied. Infection of leaves surface controlled by pre-treatment of Benomyl 4000ppm, for 20 minutes and treatment of alcohol 70% and sodium hypochlorite 1% for 15 seconds and 20 minutes respectively. Different parts of leaves have been chosen and were planted on the Pierik medium containing BA and Kin in presence of 2,4-D with various concentration. The most amount of callus produced in medium containing Kin (4 mg/l) and 2,4-D (1mg/l) in darkness. The highest number of plantlet over the callus resulted in Pierik medium with concentration of Kin (2 mg/l) and IAA (0.25 mg/l) in light condition.

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