Molecular Analysis Of The Bovine Leptin Gene In Iranian Sarabi Cattle (Iranian Bos Taurus)
2006
Javanmard, A., Agriculture Faculty, UPM, Malasia. | Elyasi Zarin Gabay, G., East Azarbaijan Research Center for Agriculture and Natural Resources. | Gharedaghi, A. K., Animal Science Research Institute of Iran. | Asadzadeh, N., Animal Science Research Institute of Iran. | Banabazi, M.H., Animal Science Research Institute of Iran. | Nasiri, M.R., Agriculture Faculty Ferdowsi University of Mashhad. | Javadmanesh, A., Agriculture Faculty Ferdowsi University of Mashhad.
There is evidence of a genetic correlation between energy balance, milk yield and state of luteal activity. Leptin is a 16 kD protein that synthesis by white adipose tissue and it involves in regulation of feed Intake, energy balance, fertility and immune function. In cattle, the leptin gene is located on chromosome four. It consists out of 3 exons and 2 introns of which only 2 exon are translated in to protein. In total 66 animals from Sarab and Shabestar Stations were genotyped for this project. A strategy employing polymerase chain reaction was used to amplify a 422-bp fragment of intron 2 from blood DNA. Digestion of amplicons with sau3AI revealed two alleles: allele A had 2 fragments 390 and 32 bp and allele B had 3 fragments 303, 88 and 32 (88 and 32 fragment have not detected on the gel). Three patterns were observed. Frequencies were 0.31, 0.43 and 0.15 for AA, AB and BB respectively. This polymorphism could be evaluated for marker assisted selection and the developed PCR method would expedite screening for large number of animals for such these studies.
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