Evaluation Of Resistance For Drought And Salinity In Four Barley (Hordeum Vulgaris L.) Ultivars In Germination Stage
2007
Heidary, M., Natural Resources Research Center of West. Azarbdjan Province, | Heidariy , R., Department of Biology, Faculty of Science, University of Uromieh, Iran. | Jameai, R., Department of Biology, Faculty of Science, University of Uromieh, Iran.
The salinity and drought are two increasing stress in region soils.So that the selection of resistant cultivars is necessary and the most important.In order to study salinity and drought resistant of barley four cultivars named Sahand , Makoii , Naghadeh and CB were selected. By using randomized complete block design seeds of all cultivars were planted. The salinity and drought levels were respectively 5,10 ,15and 20 gr/lit NaCl, ,25 ,50,100,150 gr/lit glucose.The results showed that in critical salinity (20 gr/lit NaCl) and drought (150 gr/lit) conditions the percentage germination of sahand and CB were respectively 80,27 and87,31. Then Sahand was resistant and CB sensitive cultivar. Sahand and CB were planted under field capacity F.C..Condition in three levels of F.C., 1/2 F.C.,1/4 F.C... After ten days concentration of free proline and soluble sugars in leaves and roots of seedling were determined. By increasing of drought stress, accumulation of proline and soluble sugars content leaves and roots of both cultivars were significantly increased.We experimented Alpha-amylas activity in four days seedlings that germinated under drought and salinity condition. Alpha- amylas activity in the Shand was higher than that in the CB..In electrophoresing ( SDS-PAGE ) of seedling protein of treatment under drought and salinity condition ,the most differences were in some bands with 62.7,43.35 and 28.68 KD molecular weight.
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