Control of root knot nematode(Meloidogyne spp) by using of nematicides on cucumber(Cucumis sativus) and determination of residue on fruit.

In order to study the impact and time of application of cadusafos(Rugby 10G) and phenamiphos(Nemacur 10G) for control of the root knot nematode, Meloidogyne javanica, infecting cucumber,an experiment was conducted with 7 treatments at two growing seasons under gllashouse condition: In the first experiment, the doses of nematicides used were 4,6 and 8 kg a.i./ha(3,4.5 and 6 g/m2 of commercial product) and in the 2nd experiment the doses used were 7.5,15,30 and 40 kg a.i./ha(3.75,7.5,15 and 22.5 g/m2 of commercial product) and control (check treatment) with three replications. Nematicides were applied one week before at the time two weeks after , at the time and sowing of seeds. The experiments were conducted in completely randomized block design and following parameters i.e. no.of galls and eggmasses, no. of eggs, adult, J2, final population(Pf),dry and fresh weights of shoot and root, and plant height were measured at termination of experiments. Data were analysed using SPSS software. The results of experiments revealed that there were no significant differences between the nematicides used for the control of the root knot nematode, however, both the nematicides reduced the nematode population. In the first experiment, the nematicides did not reduce nematode population significantly when compared with the control. Studies on population variations indicates that the highest population of nematodes were recovered in treatment where nematicides were applied one week before sowing. In the second experiment, the results indicate that the most effective nematicide dose used was 22.5 g/m2 and the best time of its application was one week prior to sowing of seeds. However, the results revealed that in concomitant and sequential (one week before) treatment, highest yield were obtained. For the determination of Nemacur residue of the most effective dose of 22.5 gr/m2 the products of the treatment blocks were harvested for six times. The samples were transferred to the laboratory and then prepared for extraction, purification and finally measurement of the residues by GC-NPD. The results were compared with the Codex Alimentarius MRL for Nemacur on vegetables which is 0.5 ppm and the best timing for pesticide application which was one week before sowing of seed was chosen. It is therefore suggested that the cucumbers produced up to 3th stage of harvesting be used by the consumers, because the products harvested up to this stage possess residues wich are closed to the MRL shown by codex.