Effects of acid treatments on in vitro seed culture of some turfgrass genera
2003
Salehi, Hasan, Department of Horticultural Science, College of Agriculture , Shiraz University | Khosh Khui, Morteza, Department of Horticultural Science, College of Agriculture , Shiraz University
Callus production from seed explants of turfgrasses is a primary stage for somatic embryogenesis or adventitious shoot production commonly used in biotechnological methods including gene transformation. In some of turfgrass genera low callus production is the main problem in in vitro culture of seeds. In this study, the following treatments were used: Lolium perenne L. ‘Barball’ with 50% acid for 10 min; Poa pratensis L. ‘Merion’ with 50% acid for 15 min; Festuca rubra L. var. rubra ‘Shadow’ with 25% acid for 15 min and Cynodon dactylon (L.) Pers. with 50% acid for 20 min, all with controlsin in vitro culture of seeds. Interaction between these treatments and light or dark conditions was also investigated. Each treatment consisted of 4 replications, 100 seeds per replicate. After treatment and surface sterilization, seeds were cultured on MS basal medium supplemented with 8 g l-1 agar-agar, 30 g l-1 sucrose and 150 ىM 2,4-D for Lolium and Poa, 200 ىM 2,4-D for Festuca and 40 ىM 2,4-D for Cynodon. Number of cultured seeds producing callus, was measured, 4 wk after the culturing. Results indicated that acid treatments, improved both callus production percentage and rate, compared to control. Cynodon had the highest callus production percentage and thereafter were Poa, Lolium and Festuca, respectively. Cynodon had the highest callus production rate and thereafter were Lolium, Poa and Festuca, respectively. Cynodon had the best callus production in light and the other genera in dark conditions.
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