Evidence for new beta1-3 galactosyltransferase avtivity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera
2007
Kimura, Y.(Okayama Univ. (Japan)) | Sakamura, S. | Ushijima, T. | Hama, Y. | Kajiura, H. | Fujiyama, K. | Okihara, K. | Hashimoto, K. | Sugimoto, H. | Yamada, H.
In a previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Galbeta1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new beta1-3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such beta1-3galactosyltransferase activity, which can transfer galactosyl residues with the beta1-3 linkage to beta1-4 GalNAC residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAcsub(2)GlcNAcsub(2)Mansub(3)GlcNACsub(2)-PA as acceptor N-glycan, we detected the beta1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique beta1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.
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