Comparison of regeneration efficiency of different genotypes of indica rice cultivars
2007
Abayawickrama, A.S.M.T.(Saga Univ. (Japan). Faculty of Agriculture) | Anai, T.
Establishment of high efficiency Agrobacterium-mediated transformation techniques for indica rice (Oryza sativa L.), offers the potential for the introduction of specific genes from any source into existing elite lines, for the purpose of varietal improvement. In this study, we identified optimal tissue culture conditions for callus induction and regeneration, the two major steps in the transformation process. The tissue culture conditions were tested using 15 indica cultivars and one japonica cultivar. The results of the present study revealed that callus induction was enhanced when used MS as the basal in callus induction medium. On the other hand, calli which were induced on N 6 based medium, has greatly improved the regeneration ability of indica rice. In addition, cultures which were incubated under light at 30 deg C enhanced both callus induction and regeneration ability. IR 24, K-13, K-14, K-15 and Tetep have been identified as the best varieties for transformation experiments since both callus induction and regeneration ability of these varieties were significantly high.
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