Micropropagation of plantlets through callus in taranoki (Aralia elata)
2007
Karim, Md.Z.(Tokyo Univ. of Agriculture and Technology, Fuchu (Japan)) | Yokota, S. | Rahman, Md.M. | Eizawa, J. | Saito, Y. | Azad, M.A.K. | Ishiguri, F. | Iizuka, K. | Yoshizawa, N.
In order to establish the micropropagation protocol of plantlets in Taranoki (Aralia elata), the present study was investigated callus induction from petiole and leaf segments and subsequent plantlet regeneration. The highest frequency of callus induction (96.7%) was obtained in a BT medium supplemented with 5.0 mciroM 2,4-D using petiole explants. The highest regeneration rate of adventitious shoots, number of total shoots / culture, and average length of shoots / culture from petiole-derived calli were obtained in a PGR-free BT medium. The highest frequency (100%) of rooting was achieved when they were cultured in a BT medium containing 2.0 microM NAA. Rooted shoots were acclimatized successfully and established in the soil under greenhouse conditions with a final survival rate of 72%.
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