Study of simultaneous analytical method of veterinary drugs using liquid chromatography/tandem mass spectrometry
2006
Nishikawa, T,(Nagasaki-ken. Inst. of Public Health and Environmental Sciences (Japan)) | Baba, T. | Murakami, M.
A simple method using liquid chromatography/tandem mass for simultaneous determination of veterinary drugs and their metabolites in livestock products and seafoods (bovine muscle, egg, milk and shrimp) was studied. Mass spectral acquisition was done by applying multiple reaction monitoring (MRM). The veterinary drug was extracted by acetonitrile- methanol (70: 30, v/v), and the extracts defatted by n-hexane with saturated acetonitrile. The extracts were evaporated, dissolved with acetonitrile-water (10:90) for analysis by liquid chromatography/ tandem mass. The veterinary drugs were separated by reversed-phase LC using Mightsil RP-18GP column and determined by electrospray ionization tandem mass spectrometry. The detection limits were range from 0.02 ng/g to 5.25 ng/g. The recoveries of veterinary drugs and their metabolites were almost 60-120% from livestock products and seafood respectively.
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