Temporal expression of growth factor genes of primary porcine satellite cells during myogenesis
2006
Theil, P.K.(Danish Inst. of Agricultural Sciences, Tjele (Denmark)) | Sorensen, I.L. | Nissen, P.M. | Oksbjerg, N.
The present experiment was undertaken to study the transcription of members of the insulin-like growth factor (IGF) system and myostatin in porcine satellite cells through myogenesis and to study the effects of 1 micromol/L insulin on stimulating fusion. Cultured cells were harvested at 50 and 80% confluence and at days 1, 2, 3 and 4 following induction of fusion and differentiation. Increased levels of myogenin transcription and creatine kinase from 50% confluence until day 2 indicated that fusion and differentiation were induced. The mRNA abundance of IGF-I (P0.001), IGF-II (P0.001), IGF binding protein (BP) 5 (P0.01), type I IGF receptor (P0.001) and myostatin (P0.01) increased from proliferation to differentiation. Insulin stimulated differentiation as indicated by elevated creatine kinase activity (P0.001) but did not affect myogenin mRNA abundance (P
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