Effect of propionate, pyruvate and beta-hydroxybutyric acid on pyruvate carboxylase mRNA expression of in vitro culture bovine hepatocytes
2006
Xu, C.(Jilin Univ., Changchun (China)) | Wang, Z.
The objective was to detect the effect of propionate, pyruvate and beta-hydroxybutyric acid on pyruvate carboxylase mRNA expression, in in vitro culture bovine hepatocytes through a quantity reverse transcription polymerase chain reaction method. Pyruvate carboxylase is a key enzyme in the liver gluconeogenesis of ruminants and plays an important role in the negative energy balance of peri-parturient dairy cows. The effect of propionate, pyruvate and beta-hydroxybutyric acid on pyruvate carboxylase mRNA expression at cell level is presented in this article. In experiment 1, the various concentrations of propionate from 0 mmol/L to 11.5 mmol/L, were added to the hepatocytes media. The results indicate that the level of pyruvate carboxylase mRNA was significantly enhanced accompanying increased propionate concentrations in the media (P0.01) and high concentrations of propionate did not inhibit the levels of pyruvate carboxylase mRNA. In experiment 2, pyruvate in concentrations from 0 mmol/L to 12 mmol/L was added to the media. The results indicate that the levels of pyruvate carboxylase mRNA were significantly enhanced when concentrations of pyruvate increased from 0 mmol/M to 4 mmol/M and decreased when concentrations of pyruvate increased from 8 mmol/M to 12 mmol/M (P0.01). In experiment 3, beta-hydroxybutyric acid of various concentrations from 0 mmol/L to 3.0 mmol/L was added to the media. The results indicate that pyruvate carboxylase mRNA levels increased slightly when concentrations of beta-hydroxybutyric acid were less than 1 mmol/L, and pyruvate carboxylase mRNA levels decreased significantly when they exceeded 2 mmol/L or 3 mmol/L (P0.01). There results suggest that beta-hydroxybutyric acid inhibits PC mRNA expression.
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