Improved method for bone distinction
2007
Taira, T.(Fukushima-ken. Agricultural Technology Center, Koriyama (Japan))
Grains, calcium phosphate and bone meal are feeds for non-ruminant domestic animals. However, mixtures of animal protein including bone meal are not permitted in feed for domestic ruminants to prevent the outbreak of bovine spongiform encephalopathy. This examination was carried out to distinguish bone meal from grains and calcium phosphate. Bones are composed of calcium phosphate and protein. Protein is detectable with ninhydrin. In this examination, ninhydrin dyed particles of domestic animal bones a bluish-violet color. These particles generated bubbles when added to hydrochloric acid. Ninhydrin dyed particles of grain, however, did not generate bubbles when added to hydrochloric acid. Ninhydrin did not dyed calcium phosphate. Therefore, domestic animal's bones could be distinguished from grains and calcium phosphate by using ninhydrin and hydrochloric acid.
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