Evaluation of parasite intensity and infection method with Amyloodinium ocellatum
2007
Yokoyama, F.(Nagasaki-ken. Inst. of Fisheries (Japan)) | Takami, I.
Amyloodinium ocellatum is a harmful parasite of aquarium and land-based aquaculture. Mass mortality of cultured marine fishes caused by A. ocellatum in land-based aquaculture has often occurred in Nagasaki prefecture. It is required to develop the technique of prevention and control of A. ocellatum. Thus, we aimed to establish the appropriate method of experimental infection, cyst collection and counting of the parasite on the gills. Infection of A. ocellatum was easily achieved either by cohabitation with a diseased fish or by exposure to dinospores. Partly purified cysts were collected by hanging the infected gill in 1/3 seawater for 10 min. Number of trophonts on the gills was determined by the following method; fix the infected gills in 10% formalin dissolved in 1/3 seawater, stir the gills for 5 min with a magnetic stirrer at 1,000 rpm, pass the parasite suspension through plankton net with openings of 335 microm, centrifuge the filtrate at 1,500 rpm for 5 min, resuspend the pellet in 10 ml of the fixative, stain with Lugol's solution, and count the number of trophonts under the stereoscopic microscope.
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