Effect of synthetic jasmonic acid derivatives on shoot formation in rhizome cultures of Cymbidium species
2007
Wang, T.(Ehime Univ., Matsuyama (Japan)) | Shimasaki, K. | Fukumoto, Y. | Pan, X. | Yang, X.
Lower concentrations of synthetic jasmonic acid derivatives enhanced shoot formation from rhizome segments of terrestrial Cymbidium species in vitro. Number of shoots developed from rhizome cultures of C. kanran was increased by the addition of jasmonic acid derivatives at concentrations less than 0.1microM. Shoot formation in C. ensifolium was promoted by the addition of jasmonic acid derivatives. Methyl jasmonate (Me-JA), methyl dihydro jasmonate (MDJ) and n-propyl dihydrojasmonate (PDJ) were more effective in increasing the shoot number developing from rhizome culture of C. kanran. PDJ was effective for increasing the shoot number in rhizome cultures of C. ensifolium. In C. kanran cultures, higher rates (70%) of shoot formation were obtained by treatments of PDJ (0.01-0.1microM) and MDJ (0.001-0.01microM). Higher rates (85%) of shoot formation of C. ensifolium cultures were obtained by treatment with jasmonic acid derivatives. Some shoots developed roots following treatment with jasmonic acid derivatives. Rate of rooting in C. kanran rhizomes was highest (15%) at 0.001 and 0.01microM MDJ. The rooting rate in C. ensifolium rhizomes was highest (20%) at 0.1 and 1microM CSJ.
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