Development of compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella (Dinophyceae)
2007
Nishitani, G.(National Research Inst. of Fisheries and Environment of Inland Sea, Hatsukaichi, Hiroshima (Japan)) | Nagai, S. | Masseret, E. | Lian, C. | Yamaguchi, S. | Yasuda, N. | Itakura, S. | Grzebyk, D. | Berrebi, P. | Sekino, M.
In this study, we identified 9 polymorphic compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella, which was isolated from Thau lagoon (France, Mediterranean Sea), using the compound microsatellite marker technique. These new microsatellites were characterized by screening DNA templates from 43 A. catenella clonal strains, which were collected from a seawater sample from Inokushi Bay (Oita Prefecture, Japan). These loci provide one class of highly variable genetic marker: the number of alleles ranged from 3 to 8, and the estimate of gene diversity varied between 0.285 and 0.762. We also analyzed the same 43 DNA samples using microsatellite markers previously identified for A. catenella, comparing the PCR amplification success, the number of alleles and gene diversity. These three parameters were not significantly different, showing that the compound microsatellite markers have the same potential to reveal A. catenella genetic structure. This simple and efficient method reduces the costs for developing SSR markers and for analyzing the genetic structure of populations, therefore, suggesting the effectiveness of applying this method to other species.
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