Effects of sucrose and boric acid concentration, and incubation temperature on germination of Japanese pepper (Zanthoxylum piperitum (L.) DC.) pollen on artificial media
2008
Maeda, T.(Wakayama-ken. Research Center of Agriculture, Forestry and Fisheries, Aridagawa (Japan). Fruit Tree Experiment Station) | Yonemoto, Y. | Hagiwara, S. | Taniguchi, M. | Kansako, M.
Early shipping of immature fruit of the Japanese pepper (Zanthoxylum piperitum (L.) DC.) grown under greenhouse conditions is advantageous for commanding a higher price for the fruit. However, in order to achieve a yield per unit area of fruit under greenhouse conditions, artificial pollination is required. For successful pollination, pollen with a high potential for germination must be used. Additionally identification of optimal conditions for pollen germination is also required. In this paper, we identified the optimal concentrations of sucrose, agar, and boric acid, as well as the optimal temperature for pollen germination and pollen tube length for fresh pollen of Japanese pepper at the beginning of flowering and the full bloom stage under an artificial environments. With 2% agar media, the highest percentage of germination at the beginning of flowering and at the full bloom stages were obtained at 15% and 15-20% respectively. Pollen tube length at both times excelled comparatively at 1 5-25% sucrose. The highest germination at both times was obtained at 30 deg C. Pollen tube length at the beginning of flowering and full bloom stage were comparatively higher at 20-30 deg C and 15-35 deg C, respectively. Adding 100- 1000 ppm boric acid enhanced pollen germination percentage and tube length.
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