High-Throughput DNA Extraction Method for Marker Analysis in Rice Grain
2006
Choi, Y.D. (TakaraKoreaBiomedical Inc., Seongnam, Republic of Korea) | Lee, H.K. (TakaraKoreaBiomedical Inc., Seongnam, Republic of Korea) | Lee, Y.S. (TakaraKoreaBiomedical Inc., Seongnam, Republic of Korea) | Yun, J.H. (TakaraKoreaBiomedical Inc., Seongnam, Republic of Korea) | Kim, S.J. (TakaraKoreaBiomedical Inc., Seongnam, Republic of Korea) | Park, S.W. (TakaraKoreaBiomedical Inc., Seongnam, Republic of Korea), E-mail: [email protected]
The study of molecular markers to improve crops largely depends on the availability of rapid and of efficient DNA extraction methods. Here we developed a cheap and convenient method to isolate genomic DNA from rice grains suitable for large-scale microsatellite analysis. We confirmed that the isolated rice DNA is suitable for PCR analysis with STS marker and SNP marker, as well as microsatellite marker. Further, we established high-throughput DNA extraction system in a 96-well plate format which make it possible high-throughput analysis of microsatellite markers with rice grains. This implies that the new method could be a useful tool for other types of marker analysis in large scale.
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