Cytogenetic Analysis Using Mitosis, Meiosis Chromosomes and bicolor Fluorescence in situ Hybridization of Bupleurum latissimum Nakai
2006
Kim, S.Y. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Bang, J.W. (Chungnam National University, Daejeon, Republic of Korea) | Lee, J.K. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: [email protected]
Chromosome analysis using mitosis, meiosis and bicolor FISH were carried out in Bupleurum latissimum Nakai, which is one of the endemic plants in Ulleung island of Korea. The somatic methaphase chromosomes number of this plant was 2n = 2x = 16 and the chromosome complements consisted of six pairs of metacentrics and two pairs of submetacentrics. The size of chromosomes ranged 2.40~4.20 ㎛ and NOR (nucleolus organizer region) chromosome did not observed using conventional staining.
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