Determination of Organic Acids in Tobacco Leaves by HPLC
2006
Min, H.J. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Jang, S.S. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Kim, I.J. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Shin, J.W. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Kim, Y.H. (KTnG Central Research Institute, Daejeon, Republic of Korea) | Min, Y.K. (KTnG Central Research Institute, Daejeon, Republic of Korea)
This study was carried out to improve the analytical methods for determination of organic acids in tobacco leaf by HPLC. The samples for HPLC analysis were pre-treated by means of SPE. The calibration curve for each acid was linear and R² values ranged from 0.9990 to 1.0000. The limit of detection were obtained from the calibration curves and their values were between 1.39 to 4.87 ㎍/mL. Recovery rates of organic acids were between 88.6% to 98.5%. The concentrations of organic acids among the various tobacco leaves were compared to the concentration of organic acids, were in the order oriental, burley, flue-cured tobacco.
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